RAD Microbes
Regenerative Agricultural Development

Concentrated Animal Feed
Operations — Biological
Treatment

Field-Validated Case Study Data — Hog Operations & Dairy Lagoon Management
Nature's most efficient waste processors are microscopic. When deployed correctly, microbial inoculants transform the most challenging manure management problems into demonstrable results within weeks.
Prepared by
Barry Bonner
Date
May 2026
Email
barry@radmicrobes.com
Ref
RAD-CAFO-CS-v01
radmicrobes.com

Contents

The CAFO Challenge

Concentrated Animal Feed Operations face intensifying pressure on two fronts simultaneously — operational efficiency and environmental compliance. Manure management sits at the intersection of both. Poorly managed slurry creates economic burden through labour costs, structural damage to equipment, regulatory exposure through odour and emissions, and downstream soil health consequences when over-loaded lagoon water is applied to land.

The widespread adoption of hybrid wheat straw as bedding material has compounded the problem significantly. New short-stalk wheat varieties produce thicker, denser bedding material that resists microbial degradation. The result is heavy crust formation in pull pits and lagoons — creating expanded anaerobic zones, elevated hydrogen sulphide and ammonia production, increased insect breeding environments, and substantially higher labour requirements for solids removal.

The Core Problem

Chemical inputs cannot solve a biological problem.

Conventional approaches to pit and lagoon management rely on physical removal, chemical treatment, or dilution. Each carries cost, risk, or both. The most effective and sustainable path is to restore the microbial activity that breaks down solids naturally — dramatically reducing the need for human intervention, chemical inputs, and the environmental burden placed on downstream land.

Hybrid Wheat Straw Problem

Modern short-stalk wheat varieties produce bedding with thicker cell walls and higher lignocellulose content. Standard Bacillus blends lack the cellulase enzyme activity to break this material down efficiently, causing crust to build progressively across the season.

Anaerobic Zone Consequences

As crust depth increases, anaerobic zones expand below it. These conditions drive ammonia volatilisation, hydrogen sulphide production, and VOC generation — creating welfare risks for housed animals, health risks for workers, and odour complaints from surrounding land.

Regulatory Direction of Travel

UK and EU regulatory frameworks continue to tighten around agricultural ammonia emissions and nutrient management. Operations that cannot demonstrate measurable reduction in NH3 and odour output face increasing scrutiny and potential permit conditions.

The RAD Microbes Formulation Approach

RAD Microbes worked with Aster Bio to develop a targeted biological formulation specifically designed to address the structural and biochemical challenges of modern CAFO manure management. The formulation goes beyond standard Bacillus blends by incorporating organisms selected for their performance under the specific conditions found in pull pits and aerated lagoons.

Formulation Components
Bacillus licheniformis (high cellulase strain) — Selected for exceptional extracellular cellulase production, enabling degradation of resistant hybrid wheat straw bedding. Performs under anoxic and anaerobic conditions. Produces biosurfactants that actively liquefy solids.
Pseudomonas & Paracoccus organisms — Broad-spectrum biological activity across pH ranges and temperature conditions, including the low operating temperatures encountered in winter lagoon treatment.
Multi-enzyme blend (Bacillus subtilis fermentation) — Full preservation of all enzymes produced during fermentation, standardised to target protease levels. Provides rapid-action biological initiation in high-solids systems.
Rice bran and heat-treated peat carrier — Functions as MBBR media, with pore structure supporting protected microbial growth. Enhances organism survival and colonisation in both pit and lagoon environments.
Delivery Method

The dry formulation is broadcast directly onto hog house floors and washed through slats into pull pits during routine washdown — no additional equipment, no specialist application. For lagoon treatment, product is broadcast from the shore, enabling rapid biological activation across the full water surface.

Trial Design & Comparative Testing

Formal research-grade trials were conducted in commercial hog farming operations in Russia in partnership with a specialist agricultural services company. The trials compared five treatment conditions across two testing environments — working pull pits inside live hog houses, and controlled 50-litre batch test pots — with measurements taken before treatment commencement and at the four-week point.

RAD / Aster Bio
AB S20
Proprietary Bacillus licheniformis + Pseudomonas + Paracoccus blend on peat/rice bran carrier
Competitor
Ecomic Pro B
Commercial microbial blend — Russia/international market
Competitor
Bactofor-3
Commercial microbial blend — Russia/international market
Competitor
Microzyme WT
Commercial microbial blend — USA market
Baseline
Untreated Control
No biological treatment — baseline measurement only

Measurements covered physical and chemical characteristics of slurry (suspended solids, COD, BOD5, ammonia, pH, volatile solids), atmospheric gas analysis inside barns (NH3, H2S, VOC, CH4, NO, NO2, CO2), and odour power assessment via olfactometer. Sludge depth in pits was also tracked across three application points per section.

Pull Pit Results — Working Hog Houses

The pull pit trials were conducted in live commercial hog housing. All five treatment conditions were applied simultaneously across separate sections of the same facility, ensuring consistent baseline conditions. Results are reported as percentage change from pre-treatment baseline after four weeks.

+184%
Suspended Solids
Solids converted to liquid phase — enabling effortless washdown to lagoon without manual removal
+709%
BOD5 Increase
Biological oxygen demand rise confirms active microbial digestion of converted solids in water phase
-30%
Ammonia (NH3)
Atmospheric ammonia inside barns reduced — animal welfare and worker health improvement
-56%
Volatile Organic Compounds
VOC reduction confirms anaerobic zone disruption — primary driver of odour complaints
Comparative Performance — Pull Pit Trials (% change from baseline, 4 weeks)
Parameter Control RAD / AB S20 ★ Ecomic Pro B Bactofor-3 Microzyme WT
Suspended Solids (liquefaction) Key Metric -30.3% +184.1% -32.6% +57.5% +19.1%
BOD5 (biological activity) +54.4% +709.1% +648.5% +251.6% +174.1%
NH3 Barn Atmosphere +34.1% -29.9% -15.9% -19.1% -31.5%
VOC Barn Atmosphere -11.1% -55.6% -35.7% 0% +100%
H2S (all <2.0 ppm — safe) +5.0% +166.7% +237.5% +30.0% +25.0%
COD +54.7% +720.7% +654.7% +256.9% +179.7%
NO (nitrogen oxide) -98.4% -80.0% -98.6% -94.0% -97.7%
Odour Power D/T (olfactometer) +11.0% +22.3% -23.7% -20.0% -75.7%
Interpretation

What the solids liquefaction result means in practice.

The 184% increase in suspended solids in the water phase is the headline result for operations managers. This confirms that solid crust and settled sludge is being actively converted into liquid form by microbial activity. In a pull pit system, liquid material flows freely through slats and washdown channels into downstream lagoons. Solid material does not. The RAD formulation converts the problem directly at source, eliminating the need for manual labour, additional water volumes, or mechanical agitation to move solids out of the housing environment.

H2S readings across all treatment plots remained below 2.0 ppm throughout the trial — well within safe working limits. The increases recorded reflect enhanced anaerobic activity in deeper pit zones during active solids breakdown, not a welfare or safety concern.

Batch Test Results — 50-Litre Controlled Pots

Parallel testing in controlled 50-litre batch pots allowed isolation of biological variables and more precise gas-phase measurement. Starting conditions were standardised across all five treatment plots. Results confirm the field trial data and add additional precision to odour reduction performance.

+185%
Suspended Solids
Controlled pot result — confirms field trial performance. Superior solids conversion to liquid phase.
-88%
Odour Power
Olfactometer measurement — 88% reduction in odour power. Strongest result of any product tested.
-38%
NH3 Atmosphere
Ammonia reduction in controlled environment. Consistent with barn atmospheric results.
-66%
H2S Atmosphere
Hydrogen sulphide reduction in controlled pots — distinct from barn environment where increased activity was noted.
Odour Power Comparison — Batch Test (D/T, olfactometer — lower is better)
Product Before Treatment (D/T) After 4 Weeks (D/T) Change
Control (untreated)5005000%
RAD / AB S20 Best Result50060-88%
Ecomic Pro B500400-20%
Bactofor-3500100-80%
Microzyme Waste Treat500100-80%

The batch test odour result is particularly relevant for UK operators. Planning applications for new or expanded CAFO facilities increasingly require odour impact assessments and mitigation commitments. A demonstrated 88% reduction in odour power under controlled conditions provides a strong baseline for both regulatory engagement and neighbour relations management.

Pit Sludge Level Data

Sludge depth measurements were taken at three application points within each treatment section before and after the four-week trial period. A reduction in measurable sludge depth is the expected long-term outcome of a successful biological programme driven by solids liquefaction.

Section Product Point 1 Before Point 1 After Point 2 Before Point 2 After Point 3 Before Point 3 After
Section 4.3RAD / AB S20 Lowest Final1003458154
Section 3.1Control615222035
Section 3.2Ecomic Pro B0526381010
Section 4.2Bactofor-313217160
Section 5.1Microzyme WT00204780
Note on Sludge Data

The sludge level data shows variation across measurement points that reflects the complexity of monitoring within a working hog house. The increase recorded at Point 2 in the RAD section is consistent with active solids liquefaction drawing material from adjacent pit zones into the measurement area — a function of the suspended solids conversion mechanism confirmed in the liquid phase data. A full-barn trial would provide more definitive sludge reduction figures. The suspended solids data from the liquid phase measurements is the stronger and more consistent indicator of biological activity — and RAD consistently leads all products tested on that metric.

Quebec Dairy Farm — Lagoon Management

Live Operation — Ongoing Programme
Dairy Manure Lagoon Treatment — Quebec, Canada
Same formulation as Russian hog farm trials — applied to manure treatment ponds

A dairy operation in Quebec has been running the RAD formulation in their manure treatment lagoons as part of an ongoing management programme. The operator applied the same biological blend used in the Russian hog house trials, adapted for open lagoon application in a dairy context.

Operator Reported Outcomes
Odour control — Good odour control from the lagoons throughout the treatment period, consistent with the VOC and ammonia reductions measured in the controlled trials.
Reduced solids accumulation — Visible reduction in settled solids within the treatment ponds, consistent with the solids liquefaction mechanism confirmed in the Russian trial data.
Improved field performance — Fields irrigated with lagoon water from treated ponds have shown improved plant health and growth response compared to prior seasons, attributed to improved nutrient availability in treated effluent.
"Better plant health and growth on the fields irrigated with lagoon water — and the smell from the ponds is significantly reduced."
Dairy Operator — Quebec, Canada

As biological treatment improves the nitrogen conversion and microbial profile of lagoon water, land application becomes a more effective and more controlled nutrient input rather than a waste disposal exercise. This shifts the economic framing of manure management from a cost centre to a partial nutrient recovery system.

Application for UK Hog Operations

The challenges documented in this case study are directly applicable to UK intensive pig production. UK operators using pull-pit housing systems with wheat straw bedding face identical structural biology problems to those that drove the Russian trial programme. The regulatory environment in England, Wales, and Scotland has also accelerated significantly in terms of ammonia emission obligations and nutrient management planning requirements.

Ammonia Reduction Obligations

UK National Emission Ceilings Regulations and the Gothenburg Protocol commit the UK to progressive ammonia reduction targets. Intensive pig operations are primary contributors. The 30% NH3 reduction demonstrated in live barn conditions provides a directly relevant data point for compliance planning conversations.

Nutrient Neutrality & NVZs

Operators in Nitrate Vulnerable Zones face strict controls on slurry application volumes and timings. Improving the quality and biological activity of lagoon water through microbial treatment supports more efficient nutrient use and reduces the risk of over-application events that trigger regulatory action.

Labour & Operational Cost

The conversion of pit solids to liquid phase is one of the most labour-intensive operations in pig housing management. Eliminating or substantially reducing the frequency of manual pit cleaning translates directly to measurable labour cost savings and reduced worker exposure to hazardous conditions.

Planning & Odour Management

UK planning applications for new or expanded livestock facilities require odour impact assessment and documented mitigation strategies. An 88% reduction in odour power under controlled conditions, and a 56% reduction in VOCs in live barn atmosphere, represents a strong evidential base for planning documentation and community engagement.

RAD Microbes UK Proposition

A biological solution with a documented evidence base.

RAD Microbes is positioned to bring the Aster Bio formulation to UK CAFO operators with a clear value proposition: field-validated biological treatment that reduces solid waste management burden, cuts atmospheric ammonia and odour output, supports regulatory compliance, and improves the agronomic value of treated lagoon water applied to land. This is a documented outcome from a comparative trial programme conducted under commercial operating conditions.

Begin a Conversation

RAD Microbes welcomes enquiries from UK pig producers, farm managers, and agricultural advisors interested in understanding whether a biological treatment programme is appropriate for their operation. The starting point is always a site-specific conversation — understanding your housing system, your current management approach, your regulatory position, and what measurable outcomes matter most to you.

There is no obligation at the enquiry stage. We will review your situation and give you an honest assessment of what a biological programme can and cannot deliver for your specific context.

Get in Touch
Ready to discuss your operation?
Contact
Barry Bonner
Founder, RAD Microbes
Email
barry@radmicrobes.com
Phone
+1 415 515 1665
Website
radmicrobes.com
RAD Microbes is headquartered in Dallas, Texas with molecular genomics laboratory in Houston. UK and international enquiries are handled directly by Barry Bonner.
Data Source: Trial data presented in this document was generated by Aster Bio in partnership with a specialist Russian agricultural services company during 2020 field trials. Results represent a single trial cycle across multiple treatment plots within live commercial hog operations and controlled batch testing. Additional trials and site-specific assessments are recommended before programme implementation. The Quebec dairy farm data is based on operator-reported observations and has not been independently verified. RAD Microbes — radmicrobes.com